Direct Assay for Progesterone in Saliva: Comparison with a Direct Serum Assay
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چکیده
منابع مشابه
A direct 3H-radioligand assay for serum progesterone compared with an assay involving extraction of serum.
The direct assay of serum progesterone after denaturation of the binding proteins was investigated. 50ul of patients' serum was diluted with 750ul phosphate buffer (0.05M, pH 7.4) and heated to 65 degrees C for 20 minutes. After cooling, 300ul of the treated serum was reacted with a rabbit antiserum to progesterone-11 alpha-hemicuccinyl-bovine serum albumin conjugate (Bioclin, U.K) and 1,2,6,7,...
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ژورنال
عنوان ژورنال: Annals of Clinical Biochemistry: International Journal of Laboratory Medicine
سال: 1985
ISSN: 0004-5632,1758-1001
DOI: 10.1177/000456328502200605